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KMID : 1134820210500030294
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Journal of the Korean Society of Food Science and Nutrition
2021 Volume.50 No. 3 p.294 ~ p.300
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Optimization of Ultrasound-Assisted Extraction for Triterpene Compounds from Centella asiatica Using Response Surface Methodology
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Seong Eun-Jeong
Heo Hui-Jin
Oh Se-Won
Kim Dae-il
Jang Keum-Il
Lee Jun-Soo
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Abstract
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Centella asiatica is a psychoactive medicinal plant belonging to the Apiaceae family. The major bioactive compounds of Centella asiatica are triterpene saponins, including madecassoside, asiaticoside, madecassic acid, and asiatic acid. This study aimed to optimize the ultrasound-assisted extraction conditions to extract triterpenes from Centella asiatica using response surface methodology. A Box-Behnken design was employed to develop the following three extracting parameters, methanol concentration (X1: 60∼100%), extraction time (X2: 10∼50 min), and ultrasonic power (X3: 150∼250 W). The optimal extraction conditions of madecassoside (83.14 mg/g dry weight) and asiaticoside (19.71 mg/g dry weight) were obtained at X1=80%, X2=30 min, and X3=200 W. The predicted values from the model matched well with the experimental results obtained using the optimal extraction conditions. The coefficients of variation for the inter-day and intra-day precisions were less than 5%. The limits of detection for madecassoside, asiaticoside, madecassic acid, and asiatic acid were 0.32, 0.71, 0.09, and 0.15 μg/mL, respectively. These results indicate that the validation parameters were generally reliable and satisfactory.
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KEYWORD
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optimization, ultrasound-assisted extraction, Centella asiatica, response surface methodology, triterpene
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